綠螢光蛋白(GFP)探索實驗組

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搭配實驗手冊

• 包含數量: 6組 (每組包含4個實驗模組)
• 所需時間: 3hr15min
• 包含內容:

Instructions, BactoBeads™ ampicillin, CaCl₂, pGFP plasmid, growth additive, bottle of recovery broth, Lysozyme, TEG buffer, column elution buffer, dry molecular sieve matrix, control cell extract containing GFP, protein molecular weight standards, protein denaturing solution, 50% glycerol solution, 10x gel loading solution, Tris-Glycine-SDS electrophoresis buffer (10x), Protein InstaStain®, 2 bottles of ReadyPour™ luria broth agar, petri plates, plastic microtipped transfer pipets, wrapped 10mL and 1mL pipets, inoculating loops, microcentrifuge tubes, microtiter strips, and chromatography columns.

• 需搭配設備:

微量吸管(5~50ul)、2個水浴槽、浮板架、溫度計、培養箱、冰桶和冰、標記筆、膠帶、長波UV燈、UV防護鏡、吸管幫浦(或吸球)、微波爐、離心機、冰箱(-20˚ C)、鐵環架與管柱夾具、電泳設備、聚丙醯安凝膠、電源供應器、秤量托盤、搖晃平台、冰醋酸、酒精、蒸餾水

 (部分試劑與樣品與冷藏或冷凍保存，詳見實驗手冊)

轉形作用(Transformation)

細菌培養

管柱層析法

蛋白質電泳

SDS-PAGE電泳