綠螢光蛋白(GFP)的大腸桿菌轉形作用

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搭配實驗手冊

• 包含數量: 10組
• 所需時間:
  • 設置: 50min
  • 培養: 隔夜(約12hr)
  • 轉形: 15min
• 套組包含: 

Instructions, BactoBeads™ E. coli GFP Host, supercoiled pFluoroGreen™ plasmid DNA, ampicillin, IPTG, CaCl₂, Growth Additive, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic microtipped transfer pipets, wrapped 10 ml pipet (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes

• 需搭配設備

微量吸管 (5-50 µl) 、兩個水浴槽 (37ºC and 42ºC)、溫度計、培養箱 (37ºC)、冰塊、標記筆、本生燈、加熱板(或微波爐)、隔熱手套、長波UV燈

    (部分試劑與樣品與冷藏或冷凍保存，詳見實驗手冊)

轉形作用(Transformation)

基因工程(Gene Construction)

克隆(Clone)

細菌生物學